During the TCA cycle, at which step does substrate-level phosphorylation occur?

Biochemistry MCQs for PPSC, FPSC, NTS, and Pakistan government job tests. Select an option below, then read the explanation.

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Subject
Biochemistrymedical-mcqs › biochemistry
Published
15 Jul 2019
Last updated
28 May 2026

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Explanation

Substrate-level phosphorylation in the TCA cycle happens specifically during the conversion of succinyl CoA to succinate, where a high-energy thioester bond is used to generate GTP (or ATP). Other steps involve oxidation or rearrangement but do not produce ATP directly.

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